5-ELISA ACE 2 Antigen
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5-ELISA ACE 2 Antigen

Code: A5D-55901
Manufacturer’s Part Number: 5D-55901
Product size: 96 tests micro-ELISA plate
Clearance: RUO

Price: $625.00

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Product Description

This sandwich ELISA is designed with polyclonal antibodies coated onto the plate for capturing soluble forms of Angiotensin Converting Enzyme 2 (ACE2) in the tested sample. Following a washing step, captured ACE2 is tagged with aperoxidase-labelled polyclonal antibody, which binds onto its free epitopes in a dose-dependent manner. After washing away the excess of immunoconjugate, the substrate, 3,3',5,5' Tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) is introduced and a blue color develops, which turns yellow when the reaction is stopped with sulfuric acid. This color is measured at 450 nm, and is directly proportional to the amount of ACE2 present in the tested sample. Results are expressed in ng/ml, by reference to a plasma calibrator, supplemented with recombinant ACE2, and established against an internal reference preparation.

ACE2, is a cell surface receptor, present on many cell types. It lowers blood pressure by converting angiotensin II (a vasoconstrictor) to angiotensin 1-7, a vasodilator. It is a major player of the Renin-Angiotensin-Aldosterone System (RAAS). ACE2 is also present in the soluble form in blood circulation, as the result of its extracellular domain cleavage by ADAM 17, releasing it from cell surface. The soluble form is essential for cleaving angiotensin II and lowering blood pressure. Increased concentrations of soluble ACE2 can be associated to various cardiovascular diseases.

Product Characteristics

Product Components

  • 1. COAT: Micro ELISA plate, containing 12x8 well strips, coated with a polyclonal antibody to human ACE 2, stabilized; the plate is packed in an aluminum pouch sealed in presence of a desiccant.
  • 2. SD: 2 vials of 30 mL Sample Diluent containing aggregated rabbit IgGs, ready to use.
  • 3. WS: 50 mL vial of 20-fold concentrated Wash Solution.
  • 4. CAL: 2 vials of lyophilized Plasma Calibrator, already diluted 1:5 when restored with 2 mL of SD*.
  • 5. C1 (High): 2 vials of lyophilized Control Plasma High, already diluted 1:5when restored with 1 mL of SD*.
  • 6. C2 (Low): 2 vials of lyophilized Control Plasma Low, already diluted 1:5when restored with 1 mL of SD*.
  • 7. IC: 500 µL vial of Anti-(h)-ADAMTS-13-HRP Conjugate, 50-foldconcentrated.
  • 8. ICD: 25 mL of Conjugate Diluent, ready to use.
  • 9. TMB: 26 mL of Peroxidase Substrate, 3,3',5,5'-Tetramethylbenzidinecontaining hydrogen peroxide. Ready to use.
  • 10. SA: 7.5 mL vial of 0.50M Sulfuric Acid, ready to use.
  • *The exact ACE 2 concentrations are indicated for each lot on the flyer included in the kit.

Product Applications

The 5-ELISA ACE2 Antigen kit is a two-site enzyme immunoassay for measuring Angiotensin Converting Enzyme 2 (ACE2) antigen in plasma or in any biological fluid where it must be tested. Research Use Only. Not for use in diagnostic procedures.

Instructions For Use

Material Safety Data Sheet (MSDS)

Data Sheet/Technical Data

Certificate of Analysis (COA)

Other Documents