Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphate Buffer (10 tablets)
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Immobilized Metal Ion Affinity Chromatography (IMAC) Phosphate Buffer (10 tablets)

Code: A09-1010-10
Manufacturer’s Part Number: 09-1010-10
Product size: 10 tablets
Clearance: RUO
Price: $80.00
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Product Description

Medicago AB Products Are Available Worldwide.

For Research Use Only.

Immobilized Metal Ion Affinity Chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Medicago's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method.

IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized wateryields a 300 mM Sodium chloride, 50 mM Sodium phosphate buffer with pH 8.0 at 22°C.

Product Characteristics

  • Exactly pre-weighed tablets in bottles and blister packs.
  • Mix elution buffer with phosphate buffer to achieve desired imidazole concentration.
  • Ultrapure, low UV280 absorbance imidazole.
  • Formulated from analytical grade chemicals.
  • Ready to use in minutes.

Product Components

  • Chemicals: Analytical grade.
  • Format: Exactly pre-weighed tablets.
  • Composition: 300 mM Sodium Chloride, 50 mM Sodium Phosphate.
  • Volume:
    Phosphate Buffer: 400 mL.
    Elution Buffer: 100 mL
  • pH: 8.0 ± 0.05 at 22°C.
  • Shelf Life:
    Phosphate Buffer: 5 years after production date.
    Elution Buffer: 3 years after production date.
  

Product Applications

Purification of histidine-tagged and non-histidine-tagged recombinant and native proteins using IMAC chromatography.

The phosphate buffer may be used for other types of affinity chromatography methods.

Instructions For Use

Material Safety Data Sheet (MSDS)

Data Sheet/Technical Data

Certificate of Analysis (COA)

Other Documents