HIT Assays

HIT Assays and Controls products are available worldwide

Enzyme Immuno-assay designed for measuring heparin-dependent antibodies of the IgG isotype, in human plasma or serum, or in any biological fluid where these antibodies must be measured. This assay measures only the IgG isotype, reported as the most associated with the clinical complications of heparin dependent antibodies (HIT/HITT), allowing confirmation of the diagnosis of HIT/HITT or its clinical suspicion. However, some cases associated with only IgM and/or IgA isotypes can be missed.

Assay characteristics	Kit components	Applications
Total Assay Time:                                                     2h15 Cut-off:                                                             A450 > 0.30 Dynamic Range:                                            A450 up to 3.0 Intra-Assay CV:                                                      4 - 6% Inter-Assay CV:                                                     5 – 8%	12x8 test kit 2x HIA Sample Diluent 3x HIA IgG positiveControl 3x Negative control 3x Platelet lysate 3x Anti-IgG (Fcg)-HRP IC 1x Conjugate Diluent 1x Wash Solution 1x TMB 1x 0.45M Sulfuric Acid	Clinical suspicion of HIT during heparin therapy. Heparin-dependent antibodies of the IgG isotype are better associated with the clinical diagnosis of HIT. The ZYMUTEST HIA IgG assay offers then a better specificity for the clinical complication of HIT, but it has less sensitivity as cases associated with only IgM and/or IgA isotypes are missed.

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