One Parameter Cytotoxicity Test Kits

IN CYTOTOX products are available worldwide

MTT test principle

Viable cells depend on an intact mitochondrial respiratory chain and an intact mitochondrial membrane. Toxic agents can be identified using mitochondrial dehydrogenases from viable cells.

The MTT assay is usually performed with adherent cell cultures using a culture medium free of phenol red and of serum.

MTT I solution is a supra-vital dye that is metabolized by the cells (incubation period 3 hours).

MTT is a tetrazolium salt (yellowish) that is cleaved to formazan crystals by the succinate dehydrogenase system which belongs to the mitochondrial respiratory chain, and is only active in viable cells. The mitochondrial succinate dehydrogenase reduces the MTT crystals into purple formazan in the presence of an electron coupling reagent. The more viable cells are present in a well, the more formazan dye is produced.

After solubilization of the MTT crystals with the solubilization solution MTT II, the amount of dyeis measured spectrophotometrically at 540 nm.

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