Four Parameter Cytotoxicity Test Kits

IN CYTOTOX products are available worldwide

Applications: Combined colorimetric assays for the quantification of the membrane integrity, mitochondrial metabolism, lysosomal integrity and activity, and total protein synthesis rate of cells in response to pharmaceutical, chemical and environmental compounds, and nutrients. Principle: This kit allows to measure sequentially four cytotoxicity parameters in one single cell culture: Membrane integrity (LDHe: Extracellular Lactate Dehydrogenase), mitochondrial activity (XTT: Tetrazolium Hydroxide), lysosomal integrity and activity (NR: Neutral Red), and total protein synthesis rate (SRB: Sulforhodamine B).  Released LDH, a marker for cell damage, is determined kinetically in the medium (NADH consumption). XTT is reduced in the cells to formazan by mitochondrial succinate dehydrogenase. The reduction rate is measured and correlates with mitochondrial activity. The neutral red (NR) assay procedure is based on the ability of viable cells to incorporate and bind neutral red within lysosomes. The fixed SRB dye, measured photometrically after solubilization, correlates with total protein synthesis rate and therefore with cell proliferation.  Unlike some other LDHe assays, the In Cytotox LDHe assay measures the oxidation of NADH to NAD+ and the concurrent reduction of pyruvate to lactate. By providing an excess of pyruvate in the reaction mixture, the In Cytotox LDHe assay is therefore insensitive to pyruvate in the culture medium, which can cause product inhibition of the reverse reaction implemented in other LDHe assays.

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