Clotting method

HEMOCLOT Factor V-L (CE Marked)
For Research Use Only in the United States and Canada

Clotting, Deficient Plasmas, & Chromogenic Assays products are available worldwide

Variant assay for measuring the Resistance of Factor V (Factor V-Leiden is resistant) to its inactivation by Activated Protein C (APC), in human plasma, using a clotting method. Assay fully designed with purified proteins and enzymes. It offers a well defined identification of heterozygous or homozygous patients carrying the factor V-Leiden mutation. The clotting time of diluted plasma is assayed with or without APC, and the ratio is calculated. Normal plasmas have a ratio > 2.00.

Assay characteristics

Kit components

Applications

Total Assay Time:                                              < 10 min.

Detection Threshold:                                               ratio= 2

Dynamic Range:                                         ratio= 1 to >2.5

Intra-Assay CV:                                                        < 3%

Inter-Assay CV:                                                        < 5%

Number of tests:                                  2x20 tests (ACK061L)
                                                        4x20 tests (ACK061K)

R1: Clotting mixture

R2A: Purified h FX

R2B: Purified h FX, containing h APC

R3: Purified h FIXa

Measurement of FV-L by its resistance to the action of Activated Protein C (APC).

Clotting time is measured without or with APC.