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The ZYMUTEST HIA, IgGAM ELISA kit, IgG Isotype, or IgG-IgM-IgA Isotype is a standardised and optimised enzyme immuno-assaydesigned for measuring global heparin-dependent antibodies of the IgG or IgM or IgA isotype, in human plasma or serum, or in any biological fluid where these antibodies must be measured.
ASSAY PRINCIPLE:
The diluted assayed plasma sample or biological fluid is introduced into one of the microwells of the coated plate, in the presence of platelet lysate. When present, heparin-dependent antibodies, of the IgG or IgM or IgA isotype, bind to biologically available unfractionated heparin, immobilised and saturated. Following a washing step, bound antibodies are revealed with the immunoconjugate, containing 3 goat polyclonal antibodies anti-human IgG (Fcg specific)- IgM (m)- IgA (a)-peroxidase (HRP) conjugate. This immunoconjugate reacts specifically and homogeneously with IgG, IgM and IgA isotypes. Following a new washing step, the peroxidase substrate, Tetramethylbenzidine (TMB) in presence of hydrogen peroxide (H2O2), is introduced and a blue colour develops. The colour turns yellow when the reaction is stopped with sulfuric acid. The colour developed is directly proportional to the amount of heparin-dependent antibodies, of the IgG, IgM or IgA isotype, present in the tested sample.
HIT Assays and Controls Products
Hyphen BioMed Information
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