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The traditional Ames test is the most generally used and validated bacterial reverse-mutation test. The test employs several mutant strains of Salmonella typhimurium, carrying mutations in the operon coding for histidine biosynthesis. When these bacteria are exposed to mutagenic agents, under certain conditions reverse mutation from histidine auxotrophy to histidine prototrophy occurs.
The increasing number of compounds to be screened and the fact, that new compounds are produced only in very small scale at this stage, were among the reasons to develop an alternative screening test to the standard Ames test.
The Ames II™ Mutagenicity Assay, based on the same principle as the traditional test, sets a new standard for this type of testing, offering several advantages over the traditional Ames and the Mini Ames tests.
The Ames II™ Mutagenicity Assay, available through Xenometrix by Endotell GmbH, is a liquid microplate modification of the Ames test which offers a higher speed format, new strains, colorimetry, easy handling and the possibility of automated plating and plate reading. The assay is fast and efficient, consumes a lower amount of test chemical than the Mini Ames test, shows good correlation with the traditional assay and was developed in the Bruce Ames laboratory at U.C. Berkeley (1). Due to the possibility of automatization, hundreds of substances can be run within a month.
Ames MPF™ 98/100, MPF™1535, MPF™1537 and Ames II™ Products
Xenometrix Information
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