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The sandwich type Enzyme-Linked ImmunoSorbent Assay (ELISA) from U-CyTech is a simple and sensitive assay for the determination of cytokine levels in biological fluids. The assay is conducted in antibody-coated microtiter plates where serial dilutions with unknown concentrations of cytokines are brought into the wells of the plate. The bound cytokine is then allowed to associate with a biotinylated detector antibody and an enzyme-labeled streptavidin polymer. Subsequently, a chromogenic substrate is introduced producing a colored product that can be determined spectrophotometrically.
U-CyTech has developed various ELISA kits for the detection of rat cytokines in cell culture supernatant, plasma or serum. To obtain kits with high sensitivity and accuracy, it is U-CyTech's policy to develop and select 'in house' specific mono- and polyclonal antibodies with high affinity for a range of rat cytokines. The validated compatibility of these antibodies with certain high-quality ancillary reagents and plates guarantees trouble-free operation and optimal results.
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